Mouse Transgenic Facility

Coordinates 50.813240674624815, 4.3822972998418415

Contact name Vanessa Depaepe

CIVIS Label No

Acronym MTF

Scientific domain

LS4_12 - Cancer - Physiology in Health, Disease and Ageing

LS4_1 - Organ and tissue physiology and pathophysiology - Physiology in Health, Disease and Ageing

LS3_2 - Cell senescence, cell death, autophagy, cell ageing - Cellular, Developmental and Regenerative Biology

LS6_6 - Infectious diseases - Immunity, Infection and Immunotherapy

LS5_12 - Mental disorders - Neuroscience and Disorders of the Nervous System

LS4_3 - Physiology of ageing - Physiology in Health, Disease and Ageing

LS1_1 - Macromolecular complexes including interactions involving nucleic acids, proteins, lipids and carbohydrates - Molecules of Life: Biological Mechanisms, Structures and Functions

LS1_6 - Glycobiology - Molecules of Life: Biological Mechanisms, Structures and Functions

LS1_5 - Lipid biology - Molecules of Life: Biological Mechanisms, Structures and Functions

LS5_11 - Neurological and neurodegenerative disorders - Neuroscience and Disorders of the Nervous System

LS6_5 - Biology of pathogens (e.g. bacteria, viruses, parasites, fungi) - Immunity, Infection and Immunotherapy

LS9_3 - Bioengineering of cells, tissues, organs and organisms - Biotechnology and Biosystems Engineering

LS1_9 - Molecular mechanisms of signalling processes - Molecules of Life: Biological Mechanisms, Structures and Functions

LS5_10 - Ageing of the nervous system - Neuroscience and Disorders of the Nervous System

LS5_8 - Neural basis of behaviour - Neuroscience and Disorders of the Nervous System

LS5_9 - Neural basis of cognition - Neuroscience and Disorders of the Nervous System

LS5_7 - Sensory systems, sensation and perception, including pain - Neuroscience and Disorders of the Nervous System

LS5_5 - Neural networks and plasticity - Neuroscience and Disorders of the Nervous System

LS3_5 - Cell signalling and signal transduction, exosome biology - Cellular, Developmental and Regenerative Biology

LS3_6 - Organelle biology and trafficking - Cellular, Developmental and Regenerative Biology

LS3_3 - Cell behaviour, including control of cell shape, cell migration - Cellular, Developmental and Regenerative Biology

LS3_8 - Embryogenesis, pattern formation, morphogenesis - Cellular, Developmental and Regenerative Biology

LS3_4 - Cell junctions, cell adhesion, the extracellular matrix, cell communication - Cellular, Developmental and Regenerative Biology

LS4_10 - The cardiovascular system and cardiovascular diseases - Physiology in Health, Disease and Ageing

LS4_5 - Non-hormonal mechanisms of inter-organ and tissue communication - Physiology in Health, Disease and Ageing

LS4_13 - Other non-communicable diseases (except disorders of the nervous system and immunity-related diseases) - Physiology in Health, Disease and Ageing

LS5_1 - Neuronal cells - Neuroscience and Disorders of the Nervous System

LS5_4 - Neural stem cells - Neuroscience and Disorders of the Nervous System

LS5_6 - Neurovascular biology and blood-brain barrier - Neuroscience and Disorders of the Nervous System

LS5_14 - Repair and regeneration of the nervous system - Neuroscience and Disorders of the Nervous System

LS3_7 - Mechanobiology of cells, tissues and organs - Cellular, Developmental and Regenerative Biology

LS3_1 - Cell cycle, cell division and growth - Cellular, Developmental and Regenerative Biology

LS3_13 - Stem cells - Cellular, Developmental and Regenerative Biology

LS3_14 - Regeneration - Cellular, Developmental and Regenerative Biology

LS1_4 - Protein biology - Molecules of Life: Biological Mechanisms, Structures and Functions

LS5_2 - Glial cells and neuronal-glial communication - Neuroscience and Disorders of the Nervous System

LS1_8 - Structural biology - Molecules of Life: Biological Mechanisms, Structures and Functions

LS1_3 - DNA and RNA biology - Molecules of Life: Biological Mechanisms, Structures and Functions

LS3_11 - Evolution of developmental strategies - Cellular, Developmental and Regenerative Biology

LS3_10 - Developmental genetics - Cellular, Developmental and Regenerative Biology

LS4_6 - Microbiome and host physiology - Physiology in Health, Disease and Ageing

LS5_13 - Nervous system injuries and trauma, stroke - Neuroscience and Disorders of the Nervous System

LS5_15 - Neuroimmunology, neuroinflammation - Neuroscience and Disorders of the Nervous System

LS6_1 - Innate immunity - Immunity, Infection and Immunotherapy

LS6_9 - Antimicrobials, antimicrobial resistance - Immunity, Infection and Immunotherapy

LS9_1 - Bioengineering for synthetic and chemical biology - Biotechnology and Biosystems Engineering

LS4_2 - Comparative physiology - Physiology in Health, Disease and Ageing

LS6_7 - Mechanisms of infection - Immunity, Infection and Immunotherapy

LS4_4 - Endocrinology - Physiology in Health, Disease and Ageing

LS6_3 - Regulation of the immune response - Immunity, Infection and Immunotherapy

LS6_2 - Adaptive immunity - Immunity, Infection and Immunotherapy

LS6_4 - Immune-related diseases - Immunity, Infection and Immunotherapy

LS5_3 - Neural development and related disorders - Neuroscience and Disorders of the Nervous System

LS9_2 - Applied genetics, gene editing and transgenic organisms - Biotechnology and Biosystems Engineering

LS4_9 - Metabolism and metabolic disorders, including diabetes and obesity - Physiology in Health, Disease and Ageing

LS1_2 - Biochemistry - Molecules of Life: Biological Mechanisms, Structures and Functions

LS4_11 - Haematopoiesis and blood diseases - Physiology in Health, Disease and Ageing

LS3_9 - Cell differentiation, formation of tissues and organs - Cellular, Developmental and Regenerative Biology

LS6_8 - Biological basis of prevention and treatment of infection - Immunity, Infection and Immunotherapy

Keywords

Transgenesis Mouse Cryopreservation CRISPR -Cas9 ES cells Rederivation Sanitisation Revitalization Microinjection Knock-out Knock-in

Partner institution

Université libre de Bruxelles

Website No

Technical staff available No

Remote access details Open to CIVIS but not external users. The Transgenic Mouse Facility has developed most key aspects involved in generation and optimal handling of transgenic mice, including development of new mouse models (addition transgenics and ES gene targeting), as well as more standard mouse supporting services such as mouse rederivation and sperm and embryos cryopreservation procedures. All the services are accessible through the Mouse Transgenic Facility

Remote access available No

Open to external users No

Equipment A largSupport services for the use of mouse models 1. Cryopreservation of mouse embryos and / or sperm allows users to preserve mouse lines from losses associated with infections (epizootics), crossbreeding problems that some transgenic animals may present, management or genotyping errors/issues in breedings which can lead to the loss of certain lines and to reduc the maintenance costs associated with housing unused animals. 2. The rederivation of lines towards an SPF status allow users to have SPF lines, when the context of their experiment requires it, both for sending these mice to other SPF animal facilities and for carrying out experimental protocols requiring a clean immunological context. 3. The "cryo-distribution" service allows researchers to securely exchange samples cryopreserved by the Platform or by their international collaborators and to improve the efficiency of the exchange of transgenic lines. Transgenic model creation services 1. Generation of addition transgenic mice to a gain of function. 2. The generation of Knock-Out / Knock-In mice using the CRISPR / Cas9 technique Generation of chimeric transgenic mice based on a homologous recombination protocol in ES cells to target a specific locus and to generate direct or conditionnal gene invalidation e and complete description of the services provided by the CIREM’s infrastructure is available here : https://cirem.ulb.be/

Open access to updated information database No

Online booking system details On demand to Vanessa Depaepe (vdepaepe@ulb.ac.be)

Online booking system available No

Description The Platform revolves mainly around support services for the use of transgenic models (cryopreservation of mouse embryos and / or sperm, the rederivation or transfer of lines from conventional status to SPF status and line revitalization by IVF. / in vitro fertilization (IVF)) and around the services of creation of transgenic models (generation of addition transgenic mice by microinjection of zygote, Knock-Out / Knock-In mice using the CRISPR / Cas9 technique or chimeric transgenic mice (aggregation of recombinant Es cells)). The Platform can also provide its expertise to researchers during projects related to transgenesis (construction of transgenesis, implementation of the genotyping strategy for future transgenic animals, management of the colony) An additional service for the reception and transfer of living embryos has also been implemented, making it possible to work with European laboratories and avoid sending frozen material or live mice.