Platform for the Hypoxic Analysis of Cell Behavior (HYP-ACB)
Coordinates
41.9036428022199, 12.514534957176009
Contact name
Francesca Cutruzzolà
CIVIS Label
Yes
Acronym
SRI HYP-ACB
Scientific domain
LS4_9 - Metabolism and metabolic disorders, including diabetes and obesity - Physiology in Health, Disease and Ageing
LS1_2 - Biochemistry - Molecules of Life: Biological Mechanisms, Structures and Functions
Keywords
Biochemistry
Metabolism
gene expression profile
hypoxia
Partner institution
Sapienza Università di Roma
Website
No
Technical staff available
No
Remote access details
Yes. The Infrastructure is open to internal and external users.
In particular, the infrastructure is included in a European Infrastructure network called MOSBRI (Molecular Scale Biophysics Research Infrastructure) (start date 01/07/2021-30/06/2025), through which European and non-European users can ask TransNationalAccess (TNA) with support from European funding. Please visit the website https://www.mosbri.eu/ to learn how to apply for access (expenses covered by EU funding) . The amount of time allocated through MOSBRI is about 10% of the total Infrastructure available time (subjected to small variations).
Remote access available
No
Open to external users
No
Equipment
BASIC - Access by expert users (only instruments usage)
INTERMEDIATE - Access by non expert users (materials, instruments and sample handling by Facility Staff)
ADVANCED - Access by non expert users (as for INTERMEDIATE plus experimental design and data analysis by facility Staff)
Open access to updated information database
No
Online booking system details
https://drive.google.com/file/d/1f1yUcpo55S6gL-0JeB6PYP-QpO40pGS2/view?usp=sharing
Online booking system available
No
Description
The Hyp-ACB facility is a complex infrastructure able to measure, under normoxic and hypoxic conditions, the metabolic activity of living cells, with particular attention to the respiratory activity of mitochondria, in parallel with analysis of gene expression. The facility allows monitoring cell metabolism and gene expression under variable oxygen tensions, thus reproducing more closely the cell or tissue microenvironment(s) in both physiological and pathological conditions.
Understanding how metabolism and gene expression can be turned on or off in a concerted manner allows unravelling the molecular mechanisms underlying physiological metabolic homeostasis and what goes awry in disease. It will also provide information on how biological systems react to environmental stimuli. Possible applications of this technology include multiple areas of biological research, spanning from molecular oncology, cardiovascular diseases, immunology, neurodegeneration, metabolic disorders, ageing, stem cells,tissue engineering, host-pathogen interactions, toxicology, drug discovery, environmental biology, bioengineering, nutrition etc.
The Hyp-ACB laboratory includes the following key equipment :
Seahorse XFe96 Agilent
Hypoxic cabin Don Whitley mod. i2 Workstation
HypoxyLab™ Bench-top physiological oxygen incubator and workstation
Bench-top physiological oxygen incubator and workstation
Sterile hood Thermo Scientific MSC ADVANTAGE 1.2
Incubator Thermo Scientific FORMA Steri-cycle 250i
Microscope EVOS XL CORE Invitrogen
Real-Time PCR Applied Biosystems QuantStudio 3