Plateforme NeuroTimone
Coordinates
43.29236346203756, 5.359133426541125
Contact name
Michel Khrestchatisky
CIVIS Label
No
Acronym
PFNT-PTA
Scientific domain
LS5_3 - Neural development and related disorders - Neuroscience and Disorders of the Nervous System
Keywords
Advanced microscopy ; Cellular imaging ; Nanoscopy ; Super-resolution microscopy ; Molecular Interactions ; Interaction Thermodynamics ; Interactions kinetics ; Protein-target interaction ; Thermo-Stability ; Stem and iPS cells;
Partner institution
Aix-Marseille Université
Website
No
Technical staff available
No
Remote access details
PFNT services are made accessible to both the academic and private sectors.
Each request (to formulate about a consice project) shall be submitted and discussed with the scientific and technical managers of each of the PFNT components: -PFNT-NCIS : christophe.leterrier@univ-amu.fr, laure.fourel@univ-amu.fr
-PFNT-PINT : herve.kovacic@univ-amu.fr, francois.devred@univ-amu.fr ; *Microcalotimetry Pole: philipp.tsvetkov@univ-amu.fr; *Analytical ultracentrifugation Pole: pascale.barbier@univ-amu.fr, *Surface Plasmon Resonance Pole: geraldine.ferracci@univ-amu.fr, *Proteomic Analysis Pole: maya.belghazi@univ-amu.fr
-PFNT-SceNT : emmanuel.nivet@univ-amu.fr, louise.greetham@univ-amu.fr
Remote access available
No
Open to external users
No
Equipment
Among the many services provided by the NeuroTimone facility PFNT are:
-cellular imaging : super-resolution, live-cell, and high-content microscopy,
-interactome analysis: molecular interactions and termostability of biological samples,
-cutting edge services in customized stem cells model.
Open access to updated information database
No
Online booking system details
Usage regulations shared only after request for access.
Charte des Plateformes Technologiques Aix-Marseille (PTA): https://www.univ-amu.fr/system/files/2021-07/Charte%20PTF%20AMU%20M%C3%A0J21.docx.pdf
Online booking system available
No
Description
Neuroscience has become a highly technological discipline that requires expensive and complex equipments. The implementation of shared facilities allows the research community of the NeuroTimone center to have access to cutting-edge techniques and technologies to develop ambitious and globally competitive projects in different fields of research. These projects give rise to high-level scientific publications and to valorization and transfer through patents and the creation of innovative companies.
The NeuroTimone Facility PFNT was born in this framework. This infrastructure offers cutting-edge tools in biochemistry, cell biology, biotechnology, and neurobiology organized in three main components : PINT, NCIS, and SceNT:
-PINT is principally dedicated to neuroscience and neuropathology projects, but is also open to other fields of research in biology and diseases such as cancer. Each platform is under the responsability of a Scientist Manager and a dedicated Technical Manager (Engineer or Ph.D.) for technical implementation and training of users. PINT offers unique expertise in thermodynamic and kinetic analysis of molecular interactions. It is equipped with microcalorimeters (ITC and DSC), nanoDSF, spectrophotometers and an Analytical Ultracentrifuge (UCA), and two optical biosensors based on surface plasmon resonance (SPR) and two mass spectrometers. These techniques all aim to characterize molecular interactions (e.g., proteins, nucleic acids, sugars, lipids, small molecules, peptides, proteoliposomes, viral particles). They have in common the absence of the use of marked reagents, simplicity and speed of implementation. The complementarity of these techniques is widely referenced for the characterization of protein-ligand interactions. PINT is open to the academic and industrial research community and allows molecular mechanism characterization of thermodynamics and kinetics of molecular interactions, the identification/screening of ligand, targets, regulators, with an expertise in neurophysiopathology, oncology, microtubule regulation, microtubule associated proteins, and prone-to-aggregate proteins.
-The Neuro-Cellular Imaging Service NCIS is part of the Photonic Imaging NeuroTimone (PImaNT) facility. It offers access to optical microscopes for neuroscientists interested in imaging molecular and cellular processes toward better understanding of the functions and dysfunctions of the nervous system. In addition to classical widefield and confocal microscopes, NCIS provides more advanced techniques such as live-cell imaging, TIRF and super-resolution microscopy (STORM). NCIS staff also offers technical advice, help and training to taylor the imaging and analysis to your scientific question. The NCIS is located in Medicine School building on the Timone campus; microscopes are accessible to researchers for a fee after initial training on the chosen instrument.
-The Stem Cell Center NeuroTimone SCeNT consists of dedicated spaces, equipment, and competencies to support work involving human induced pluripotent stem cells (hiPSCs) and genome editing. SceNT also provides an access to equipements for cell cytometry and RT-qPCR analyses. The primary mission of SCeNT is to develop and provide cell reprograming-based tools to advance the neuroscience field by fostering basic and translational research and engaging in training for scientists to use hiPSCs for their research goals. To reach its mission, SCeNT will progressively develop an array of services available to all scientists in need of our expertise. This includes for instance (non-exhaustive list): i) hiPSC production/characterization; ii) maintenance/storage of hiPSC lines; iii) differentiation for the production of hiPSC-derivatives (on demand); iv) genome editing on hiPSCs (CRISPR-Cas9-based). In addition, SCeNT will also offer individualized training for the culture, differentiation, and reprogramming techniques specific to pluripotent human stem cells and can be consulted on experimental design, and on developing projects. Along this line, SCeNT will also function as a multi-user shared research facility dedicated to supporting work involving hiPSCs.