1. Research Infrastructures
  2. Nuclear Magnetic Resonance Platform of the Institut de Microbiologie de la Méditerranée

Nuclear Magnetic Resonance Platform of the Institut de Microbiologie de la Méditerranée

CONTACT
Coordinates 43.29236346203756, 5.359133426541125
Contact name Olivier Bornet
CIVIS Label No
Acronym NMR-IMM
Scientific domain

LS1_1 - Macromolecular complexes including interactions involving nucleic acids, proteins, lipids and carbohydrates - Molecules of Life: Biological Mechanisms, Structures and Functions


 

LS1_8 - Structural biology - Molecules of Life: Biological Mechanisms, Structures and Functions


Keywords

Nuclear Magnetic Resonance Studies


 

Structure


 

interaction and dynamic of biological macromolecules


 

metabolomics


Partner institution

Aix-Marseille Université


Website No
Technical staff available No
Remote access details The platform is open to scientific communities of both the academia and private sectors, upon request. An online process allows potential users to book the equipment in advance.
Remote access available No
Open to external users No
Equipment Into details, the 600MHz NMR spectrometer enables most heteronuclear and multidimensional NMR experiments (in 2D, 3D) to be carried out on biological samples isotopically enriched with nitrogen 15 and/or carbon 13. The cryoprobe significantly increases sensitivity, making it possible to work with low protein concentrations to avoid aggregation for example or to reduce the duration of experiments in the case of unstable proteins. This technological breakthrough proves to be very useful for metabolomic NMR and makes it possible to observe small quantities of mixture composition (of the order of micro-molar) for the study of biological composites or metabolites.
Open access to updated information database No
Online booking system details None
Online booking system available No
Description The Nuclear Magnetic Resonance (NMR) facility is located at the Institut de Microbiologie de la Méditerranée (IMM) of Aix-Marseille University. The facility is mainly dedicated to the structural determination of biological macromolecules and to the study of their interactions. It is equipped with a Bruker Avance III - 600 MHz spectrometer (superconducting magnet of 14.1 Tesla) equipped with a 5 mm TCI [1H/13C/15N/2H] cryoprobe optimised for the direct detection of proton and carbon-13.