1. Research Infrastructures
  2. Marseille Proteomics

Marseille Proteomics

CONTACT
Coordinates 43.29236346203756, 5.359133426541125
Contact name Luc Camoin
CIVIS Label No
Acronym MaP-PTA
Scientific domain

LS2_8 - Proteomics - Integrative Biology: from Genes and Genomes to Systems


Keywords

Proteome


 

Proteomics


 

Mass spectrometry


 

MS-Imaging


 

Protein characterization


 

Biomarkers screening


 

Quantitative proteomics


 

Complexomics and interactomics


 

Chemoproteomics


 

Structural Proteomics


Partner institution

Aix-Marseille Université


Website No
Technical staff available No
Remote access details MaP is open to both the academic and private sectors. Any request for the Marseille Proteomics services must be done by filling the form provided on the facility webpage or by sending an email directly to the operational manager. If you wish to request us as partners in an application for funding, we strongly advise that you contact us as soon as possible to discuss the project, so this will eventually allow us completing your application with our expertise.
Remote access available No
Open to external users No
Equipment MaP offers a wide range of services and expertise in proteomics including: -Protein characterization, -Identification by mass spectrometry PMF, MS/MS, LC-MS/MS, -Identification of protein using Edman sequencing, -Analysis of chemically (EPR-, FRET- probes, Metals…) and post-translational modifications (phosphorylation, glycosylation, ubiquitinylation, sumoylation, carbonylation, glutathionylation) by mass spectrometry, -De novo sequencing by mass spectrometry (Top down LC-MS/MS & MALDI in source decay), -Biomarkers screening, -Quantitative analysis from plasma and FFPE -Isobaric labelling and Label Free,-Biomarkers research in Cancerology and Immunology Quantitative proteomics, -Quantification using isobaric labels (iTRAQ, TMT), metabolic labelling (SILAC) and label Free, -Targeted proteomics using parallel reaction monitoring, -Complexomics and interactomics, -Biochemical approaches IP-MS, AP-MS, TaP-TaG-MS, APEX-MS and BirA-MS, -Non-covalent interactions by native ESI-MS, -Characterization by exchange H/D and MS Chemoproteomics, -Non-covalent interactions by native ESI-MS, -Characterisation by H/D exchange and MS/MS analysis, -CETSA analysis, drug affinity and click-chemistry Sample fractionation, -Off-Gel Electrophoresis of peptides and proteins, -High-pressure liquid chromatography, -1-D gels Electrophoresis Structural Proteomics, -Global mass determination of proteins by MALDI or ESI in native or denaturating conditions, -Characterization of conformers by ion mobility-MS, -Dynamics and protein zone contacts by H/D exchange and MS MS imaging, -MALDI-imaging: Characterization of biomarkers in situ and distribution of drugs in tissues Others, -Quality control of therapeutic peptides and proteins for the pharmaceutical industry, -Characterization of metabolites and pharmacokinetic studies, -Quantification of chemicals and drugs by mass spectrometry.
Open access to updated information database No
Online booking system details Charte des Plateformes Technologiques Aix-Marseille (PTA): https://www.univ-amu.fr/system/files/2021-07/Charte%20PTF%20AMU%20M%C3%A0J21.docx.pdf
Online booking system available No
Description Marseille Proteomics (MaP) is a three-site facility, whose mass spectrometry equipment offers a wide panel of complementary techniques for protein identification and characterization, biomolecule quantification, biomarker discovery, binding studies imaging, structural proteomics and bioinformatics related services. MaP is the main proteomics facility in the Région Sud; it offers collaborative and fee-for-service opportunities for basic and clinical research, as well as for the industry. MaP covers a large experimental field from oncology, immunology, and microbiology. The facility is hosted by the CRCM laboratory and labelled by both the GIS-IBiSA and Aix-Marseille University. It is equipped with a MALDI ToF/ToF UltraFlex (Bruker Daltonics), which allows the analysis of heavy molecular ions (20-30 kDa), as well as with a last-generation LC-MS/MS Orbitrap (Q-Exactive, Tribrid Fusion Lumos, ThermoFisher Electron).