Biomass Protein Engineering

Coordinates 43.29236346203756, 5.359133426541125
Contact name Marielle Bauzan
CIVIS Label No
Acronym BIOMASSE-PTA
Scientific domain

LS8_12 - Microbial ecology and evolution - Environmental Biology, Ecology and Evolution


 

LS1_1 - Macromolecular complexes including interactions involving nucleic acids, proteins, lipids and carbohydrates - Molecules of Life: Biological Mechanisms, Structures and Functions


 

LS9_4 - Microbial biotechnology and bioengineering - Biotechnology and Biosystems Engineering


Keywords

Bacteria


 

Biomass


 

Archaea


 

consortium


 

microalgae


 

cloning


 

protein expression and purification


 

1D


 

2D and 3D liquid chromatography


 

Biomolecular interaction


 

Isothermal microcalorimetry


 

biolayer interferometry


 

dynamic light scattering


 

recalcitrant protein


 

biophysical quality control


 

anerobic purification


 

bioenergy


 

bioremediation.


Partner institution

Aix-Marseille Université


Website No
Technical staff available No
Remote access details BIOMASS is open to scientific communities of both the academic and private sectors, upon request.
Remote access available No
Open to external users No
Equipment Production of microbial biomass from a commercial or isolated strain Production of extremophilic microorganisms Development of culture conditions and optimization Metabolic study. Among the services provided by BIOMASS are the production and characterization of microorganisms and resulting proteins (e.g., bacteria, archea, yeast from commercial or isolated strains, also inlcuding extremophilic microorganisms) for either molecular or metabolism studies, as well as for understanding their biomolecular interactions. These for instance include: -cloning and purification of 3D proteins under anaerobic or aerobic conditions, -quality control and biophysical/physicochemical characterization, -study of protein-protein, protein-ligand and protein-nucleic acid interactions, -study of biolayer interferometry Isothermal microcalorimetry. Assistance is provided for on each axis by a specific engineer, who is also ahead in charge of selecting the potential users' project to be implemented using BIOMASS. The 'microorganism production' axis mainly focuses on complex environmental conditions (e.g., temperature, pH, anaerobic conditions) and involves bioenergy, biomass degradation, and bioremediation processes. The 'protein' axis mainly focuses on the expression of recalcitrant proteins as well as of those from non-conventional systems. BIOMASS also offers a 3D purification service under anaerobic conditions. Finally, users may benefit from the on-site staff expertise in quality control of biomolecules (e.g., nanoparticles, liposomes...) and in biomolecular interaction.
Open access to updated information database No
Online booking system details Charte des Plateformes Technologiques Aix-Marseille (PTA): https://www.univ-amu.fr/system/files/2021-07/Charte%20PTF%20AMU%20M%C3%A0J21.docx.pdf
Online booking system available No
Description BIOMASS is hosted at the 'Institut de Microbiologie de la Méditerranée' and offers recognized technical capabilities and a unique scientific environment in the studying of fermentation processes and characterization of proteins.