1. Research Infrastructures
  2. Biomass Protein Engineering

Biomass Protein Engineering

OVERVIEW
Acronym BIOMASSE-PTA
Partner institution

Aix-Marseille Université
Description

BIOMASS is hosted at the 'Institut de Microbiologie de la Méditerranée' and offers recognized technical capabilities and a unique scientific environment in the studying of fermentation processes and characterization of proteins.

SCIENTIFIC DOMAIN
Scientific domain

LS8_12 - Microbial ecology and evolution - Environmental Biology, Ecology and Evolution





 

LS1_1 - Macromolecular complexes including interactions involving nucleic acids, proteins, lipids and carbohydrates - Molecules of Life: Biological Mechanisms, Structures and Functions





 

LS9_4 - Microbial biotechnology and bioengineering - Biotechnology and Biosystems Engineering
Keywords

Bacteria





 

Biomass





 

Archaea





 

consortium





 

microalgae





 

cloning





 

protein expression and purification





 

1D





 

2D and 3D liquid chromatography





 

Biomolecular interaction





 

Isothermal microcalorimetry





 

biolayer interferometry





 

dynamic light scattering





 

recalcitrant protein





 

biophysical quality control





 

anerobic purification





 

bioenergy





 

bioremediation.





INFRASTRUCTURE
Remote access details

BIOMASS is open to scientific communities of both the academic and private sectors, upon request.

AMENITIES
Equipment

Production of microbial biomass from a commercial or isolated strain Production of extremophilic microorganisms Development of culture conditions and optimization Metabolic study. 
Among the services provided by BIOMASS are the production and characterization of microorganisms and resulting proteins (e.g., bacteria, archea, yeast from commercial or isolated strains, also inlcuding extremophilic microorganisms) for either molecular or metabolism studies, as well as for understanding their biomolecular interactions. 
These for instance include: 

  • cloning and purification of 3D proteins under anaerobic or aerobic conditions, 
  • quality control and biophysical/physicochemical characterization, 
  • study of protein-protein, protein-ligand and protein-nucleic acid interactions, 
  • study of biolayer interferometry Isothermal microcalorimetry. 

Assistance is provided for on each axis by a specific engineer, who is also ahead in charge of selecting the potential users' project to be implemented using BIOMASS. 

The 'microorganism production' axis mainly focuses on complex environmental conditions (e.g., temperature, pH, anaerobic conditions) and involves bioenergy, biomass degradation, and bioremediation processes. 

The 'protein' axis mainly focuses on the expression of recalcitrant proteins as well as of those from non-conventional systems. 

 BIOMASS also offers a 3D purification service under anaerobic conditions. Finally, users may benefit from the on-site staff expertise in quality control of biomolecules (e.g., nanoparticles, liposomes...) and in biomolecular interaction.